Peptides

In the previous issue we published an other analysis of a IGF-1 LR3 sample we bought in the Netherlands and that originated from China.

It turned out it was a very dangerous item that contained ratinsulin instead of IGF-1 LR3!!

When we started to analyse a new sample we bought in China, we found  it was IGF-1 this time.

GHRH is a growth hormone releasing factor peptide that is comprised of either 44 or 40 amino acids and is abbreviated as GRF(1-29). The biologically active portion resides in the first 29 amino acids so the synthetic forms of GHRH are made up of just these 29 amino acids.

CJC-1295 has an added amino acid lysine which links to a drug affinity complex (DAC). CJC 1295 without DAC is modified GHRH or GRF(1-29).

Modified GRF(1-29) is a short-estered GHRH, while CJC-1295 is a long-estered GHRH. Effectively, the long-estered GHRH can be run once a week if you can find the real deal.
Since maufacturing CJC 1295 is a complex and costly process, most all CJC 1295 being currently sold is really GRF(1-29). This analysis confirmed this assumption!!

Its very profitable to buy GRF as middleman and sell them as CJC 1295-DAC

There where many discussions about on many fora about counterfeited Nordiropin Simplexx and members conpared pictures and details from their products. One of our members was convinced he had bought some counterfeits. I asked our peptide lab to run some analyses. It turned out it was  a real item.

One of our members used this petide to get a nice tan. It’s a MT2 with a nice box with hologram. The top (with brand name), label and box are made in the same colour with great precision and it looks like a pharmaceutical item. The brandname and info are not traceble on the internet, thus it concerns a underground made product, most probably originating from China or India. Our member was very curious to its quality. That’s why I decided to run an analysis.

The brandname is Scenic batchnumber: 000001380 expiring: 01 december 2010

As you can easily see, the main compound has a MW  (Molecular Weight) of 1024.5 Da (Dalton) which is in accordance with the cyclic peptide.

 

However, there are a high number of minor contaminations in the sample which you see better in the zoom pictures.

 

One of my friends, a huge guy who competes in the strongest man competition, had bought some IGF-1 vials. The brand name was Nutral with lot number 050303. According to the label it contained 1000 mcg IGF-1 LR3 in acetic acid. He lifted his shirt and showed me his body. He had blue coloured bruises alike spots on his body the size of a hand, with fluid retention underneath. Later he confessed that they had lasted for weeks. I became very curious and tried to find a laboratory that had the capability to analyse complex proteins. I found one in Germany.

THE USE OF HCG TO TREAT BENIGN PROSTATIC HYPERPLASIA

In a  published study (1), a new and potentially exciting use for hCG in men is described: the treatment of benign prostatic hyperplasia, or BPH. It has been known for some time that hCG in vitro is capable of modulating the growth of prostatic cancer cells, leading to decreased growth rates, apoptosis, and cell death. Experiments leading up to this discovery were prompted by reports that hCG is capable of blocking metastasis of neoplastic Kaposi’s Sarcoma in immunodeficient mice. Further research showed that hCG administration led to regression of Kaposi lesions in AIDS patients. Interestingly, some recent research has suggested that contaminants present in some hCG preparations, rather than the hCG itself may be responsible for the effects of hCG on Kaposi’s lesions (2). These findings have led a number of experts to recommend against the use of hCG to treat Kaposi’s sarcoma. It would be ironic if “bad” science had actually contributed to the current research suggesting that hCG is beneficial in the treatment of BPH.
 

 

“The Blue Tops”: IGF-1 LR3
by Ronny Tober

In the previous issue of Body of Science we published an other analysis of a IGF-1 LR3 sample we bought in the Netherlands and that originated from China. It turned out it was a very dangerous item that contained rat insulin instead of IGF-1 LR3! This time we are back doing protein analysis. In this case, we are again looking at IGF-1 LongR3. We obtained this vial of IGF-1LR3 from China, the principle source country of all IGF-1LR3. We did our best to pick a product that was popular, and thus was representative of the quality of many IGF-1LR3 samples. The results were, again, concerning.

The strength of blue tops from China

First I'd like to explain how the strength of Growth Hormone is measured.

First, the activity of Growth hormone was tested by using small animals, mostly rats. After much protest of organisations for animal welfare, the WHO asked some labs to seek the relationship between HPLC and the activity.  After a lot of experimenting, scientists concluded that the activity result of the RP-HPLC  (reversed phase) corresponded to the activity resulting by administration of growth hormone to small animals later these animals where killed to measure growth/strength. Then, in pharmacopoeia, the testing of activity in animals, changed to testing by RP-HPLC.