Analysis of CJC-1295 with attached Drug Affinity Complex.

GHRH is a growth hormone releasing factor peptide that is comprised of either 44 or 40 amino acids and is abbreviated as GRF(1-29). The biologically active portion resides in the first 29 amino acids so the synthetic forms of GHRH are made up of just these 29 amino acids.

CJC-1295 has an added amino acid lysine which links to a drug affinity complex (DAC). CJC 1295 without DAC is modified GHRH or GRF(1-29).

Modified GRF(1-29) is a short-estered GHRH, while CJC-1295 is a long-estered GHRH. Effectively, the long-estered GHRH can be run once a week if you can find the real deal.
Since maufacturing CJC 1295 is a complex and costly process, most all CJC 1295 being currently sold is really GRF(1-29). This analysis confirmed this assumption!!

Its very profitable to buy GRF as middleman and sell them as CJC 1295-DAC

As a general rule that you should always treat CJC 1295 as GRF (1-29). More than likely that is what you are going to be sold since it is less complicated and cheaper. He also suggest that you use 100mcg GFR (1-29); 3 times a day and combine it with 100mcg GHRP-6; 3 times a day for bodybuilding purposes.

For anti- aging DAT suggest 50mcg GFR (1-29)/100mcg GHRP-6 a day, 30 minutes before bed time.

Its analysed by HPLC-ESI-MS (HPLC 220 nm, C18 linear gradient 0-30 min). and Maldi-MS

The calculated masses:

Mass of CJC-1295 3647.17 g/mol

Mass of GHRH (1-29) 3366,88 g/mol

Mass of lysine linker 280,29 g/mol

By a purity greater then 80%

The masses found:

LC-MSD:

1684,8 amu [M+2H]2+ of GHRH (1-29)

1123,7 amu [M+3H]3+ of GHRH (1-29)

MALDI :

3365,78 amu [M+H]+ of GHRH (1-29)

3388,17 amu [M+NA]+ of GHRH (1-29)

3408,37 amu [M+K]+ of GHRH (1-29)

3432,28 amu unknown

3530,33 amu unknown

Conclusion:

The analyses sample contains the peptide  GHRH (1-29) without the lysine linker.

No significant contaminations were detectable./

GHRH is a growth hormone releasing factor peptide that is comprised of either 44 or 40 amino acids and is abbreviated as GRF(1-29). The biologically active portion resides in the first 29 amino acids so the synthetic forms of GHRH are made up of just these 29 amino acids.

CJC-1295 has an added amino acid lysine which links to a drug affinity complex (DAC). CJC 1295 without DAC is modified GHRH or GRF(1-29).

Modified GRF(1-29) is a short-estered GHRH, while CJC-1295 is a long-estered GHRH. Effectively, the long-estered GHRH can be run once a week if you can find the real deal.
Since maufacturing CJC 1295 is a complex and costly process, most all CJC 1295 being currently sold is really GRF(1-29). This analysis confirmed this assuption!!

As a general rule that you should always treat CJC 1295 as GRF (1-29). More than likely that is what you are going to be sold since it is less complicated and cheaper. He also suggest that you use 100mcg GFR (1-29); 3 times a day and combine it with 100mcg GHRP-6; 3 times a day for bodybuilding purposes.

For anti- aging DAT suggest 50mcg GFR (1-29)/100mcg GHRP-6 a day, 30 minutes before bed time.

CJC-1295 is a synthetic modification of growth hormone releasing factor (GRF) with D-Ala, Gln, Ala, and Leu substitutions at positions 2, 8, 15, and 27 respectively. These substitutions create a much more stable peptide with the substitution at position 2 to prevent DPP-IV cleavage, position 8 to reduce asparagine rearrangement or amide hydrolysis to aspartic acid, position 15 to enhance bioactivity, and position 27 to prevent methionine oxidation. By applying the Drug Affinity Complex (DAC) technology to GRF, the peptide selectively and covalently binds to circulating albumin after subcutaneous (SC) administration, thus prolonging its half-life. These substitutions are key in increasing the overall half life of CJC-1295 but there lies an even greater reason as to why the half life has been extended from ~7 minutes to greater than 7 days.

CJC-1295 is a Long acting GHRH analog. Growth-hormone-releasing hormone (GHRH), also known as growth-hormone-releasing factor (GRF or GHRF) or somatocrinin, is a 44-amino acid peptide hormone produced in the hypothalamus by the arcuate nucleus. GHRH stimulates growth hormone (GH) secretion from the pituitary. GHRH is released in a pulsatile manner, stimulating pulsatile release of GH respectively. This substance also promotes slow wave sleep. The active portion of this GRF or GHRH peptide can be found as a 29 amino acid long peptide and is appropriately named GHRH1-29. This pulsatile release of various peptides is due to the negative feedback loop that is part of the hGH axis and controls the amount of hGH that your body produces to keep it in a homeostatic environment.

Various experiments have been conducted to test the effectiveness of CJC-1295 in vivo and the Journal of Clinical Endocrinology & Metabolism has reported dose-dependent increases in mean plasma GH concentrations by 2-10 fold for more than 6 days and increased IGF-1 concentrations 1.5-3 fold for 9-11 days after a single injection.