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I`m really reading up on peptides, and these ones really interest me..
Slow Wave Sleep (SWS) enhancers
Slow Wave Sleep & Growth Hormone Night-time release are positively correlated. If you increase Slow Wave Sleep you will increase GH. Conversely it has been shown that if your Slow Wave Sleep is interrupted as is the case with sleep apnea your GH night pulse is diminished. So with that in mind here is something I wrote a few months ago on Slow Wave Sleep, GH & pGH.
SWS & GH release
There are two types of sleep, rapid eye movement (REM) and non-rapid eye movement (NREM). Sleep proceeds in cycles composed of four types of stages of NREM and a stage of REM usually ordered as: 1 > 2 > 3 > 4 > 3 > 2 > REM
The cycle lasts on average 90 to 110 minutes, with a greater quantity of stages 3 and 4 experienced early in the night and more REM later in the night.
NREM accounts for 75–80% of total sleep time. Non-REM is comprised of four stages; stages 1 and 2 are considered 'light sleep', and 3 and 4 'deep sleep' or slow-wave sleep (SWS).
It has been shown that sleep, more specifically slow-wave sleep (SWS), does affect growth hormone levels in adult men. During eight hours sleep, it has been demonstrated in several studies that the men with a high percentage of SWS (average 24%) also had high growth hormone secretion, while subjects with a low percentage of SWS (average 9%) had low growth hormone secretion.
In one very complete study referenced by several others, it was demonstrated that “GH secretory rates and peripheral GH concentrations were maximally correlated with sleep stage, with lags of 4.5 and 16 min, respectively, suggesting that maximal GH release occurs within minutes of the onset of stage 3 or 4 sleep”.
Furthermore “sleep-related augmentation of GH secretion… usually occurs around midnight and the GH levels at that time are, as a rule, at their highest during the 24-hour period. Partially, this phenomenon is time-entrained and partially related to sleep itself. It is associated with a slow wave sleep, and the maximal GH levels occur within minutes of the onset of slow wave sleep” - Holl RW, Hartman ML, Veldhuis JD, et al. Thirty-second sampling of plasma growth hormone in man: correlation with sleep stages. J Clin Endocrinol Metab 1991;72:854–61.
The origin of nocturnal GH release in humans is still unknown. Most likely hypothalamic GHRH release is a major contributing component, but an additional role of another factor, presumably augmenting GHRH responsiveness of the somatotrophs, is likely. However the precise explanatory mechanisms are still not fully identified.
It is worth reiterating though that nocturnal release of GH makes up only a fraction of the total daily GH release in women, but the bulk of GH output in men.
Delta Sleep-inducing Peptide
DSIP (Delta Sleep-inducing Peptide) is used for sleep, it also increases some Growth Hormone output and it's also being used and looked at to prevent opioid addiction.
Gaba & its derivatives
Gamma-amino butyric acid (GABA) is an amino acid and the chief inhibitory neurotransmitter in the central nervous system and the retinas of humans, which regulates muscle tone and other functions. It is also chiefly an excitatory neurotransmitter in most species.
GABA taken orally has been purported to increase the amount of the Human Growth Hormone. One such study demonstrated that gram amounts “induced clear-cut GH increments in plasma with a peak after 60–90 minutes”. The results of those studies have been seldom replicated, and have recently been in question since it is unknown whether GABA can pass the blood-brain barrier. However GABA when taken in injected form has been shown to both pass the blood-brain barrier and have a pronounced GH secretory effect.
At various times & in various studies GABA has been found to both inhibit GH release and exacerbate it. This contradiction is explained as… “inhibition of the GH release stimulated by GABA and its ability to raise baseline GH share the same basic mechanism, i.e., an action through dopaminergic (DA) neurons. Continuous stimulation of central nervous system (CNS-DA) receptors by GABA mimetics through DA release would ultimately lead to a state of partial refractoriness to DA-mediated events…”
The key then appears to be the avoidance of continuous stimulation of the central nervous system.
Two derivatives of GABA, gamma-amino-beta-hydroxy butyric acid (GABOB) and gamma- hydroxy butyric acid (GHB) have been shown to increase GH secretion as well.
PGH (aka Tyric-6)
PGH (Tyric-6) is a gaba-derivative injectable growth hormone stimulant that simply contains:
GABA - a neurotransmitter with a stabilizing role in the brain that has a calming effect. GABA also under certain circumstances increases levels of human growth hormone; and
GABOB - This has been used in the treatment of epilepsy, but it is a legal molecule that is very similar to GHB (a now illegal molecule) without GHBs negative effects; and
Magnesium amino-bromohydrate - An essential mineral which enhances the effect of GABA & GABOB.
The key to understanding why pGH (Tyric-6) is an effective GH releaser is recognizing that GHB (and presumably GABOB as well as GABA in certain circumstances) enhances both slow wave sleep and GH release. - Van Cauter E, Plat L, Scharf MB, et al. Simultaneous stimulation of slow-wave sleep and growth hormone secretion by gamma-hydroxybutyrate in normal young men. J Clin Invest 1997;100:745–53.
While GHB is illegal and not a component of PGH, its similarly structured brother GABOB has been shown to enhance slow wave sleep as well as increase GH. “Significant increases of prolactin and GH plasma levels were observed after injection with 100 mg GABOB”. - Gamma-amino-beta-hydroxy butyric acid stimulates prolactin and growth hormone release in normal women, P Fioretti,… Journal of Clinical Endocrinology & Metabolism, Vol 47, 1336-1340
In another study an intrathecal injection of 300 mg of L-GABOB to cerebrovascular patients caused significant increases in plasma GH, prolactin and cortisol levels at 60 min after injection. These results indicate that GABOB may elicit the secretion of GH, prolactin and ACTH via the central nervous system. - Stimulatory Effects of Gamma-Aminohydroxybutyric Acid (GABOB) on Growth Hormone, Prolactin and Cortisol Release in Man, J. Takahara…
Although the data is sparse and GABA studies as they relate to GH release no longer occur it appears that injectable forms of GABA & its derivatives enhance SWS (slow wave sleep) which is the time period GH is secreted. Since the majority of GH release is secreted in the first early round of SWS it seems that administration of PGH (GABA & GABOB) by injection just prior to sleep will enhance restful sleep and increase the GH secretory pulse.
Over the last few years anectdotal feedback from users including myself report both of these occurances. A protocol involving taking periodic breaks from use appears to be sufficient to promote continued effectiveness.
PGH is a simple, safe and effective compound whose ingredients may be purchased easily over the counter. GABA is sold in most supplement stores, while GABOB is harder to source but is available with international sourcing likely being less expensive.
Of course pGH is available from a research chemical facility and if purchased already assembled need only be reconstituted in Bacteriastatic Water prior to use.
Slow Wave Sleep (SWS) enhancers
Slow Wave Sleep & Growth Hormone Night-time release are positively correlated. If you increase Slow Wave Sleep you will increase GH. Conversely it has been shown that if your Slow Wave Sleep is interrupted as is the case with sleep apnea your GH night pulse is diminished. So with that in mind here is something I wrote a few months ago on Slow Wave Sleep, GH & pGH.
SWS & GH release
There are two types of sleep, rapid eye movement (REM) and non-rapid eye movement (NREM). Sleep proceeds in cycles composed of four types of stages of NREM and a stage of REM usually ordered as: 1 > 2 > 3 > 4 > 3 > 2 > REM
The cycle lasts on average 90 to 110 minutes, with a greater quantity of stages 3 and 4 experienced early in the night and more REM later in the night.
NREM accounts for 75–80% of total sleep time. Non-REM is comprised of four stages; stages 1 and 2 are considered 'light sleep', and 3 and 4 'deep sleep' or slow-wave sleep (SWS).
It has been shown that sleep, more specifically slow-wave sleep (SWS), does affect growth hormone levels in adult men. During eight hours sleep, it has been demonstrated in several studies that the men with a high percentage of SWS (average 24%) also had high growth hormone secretion, while subjects with a low percentage of SWS (average 9%) had low growth hormone secretion.
In one very complete study referenced by several others, it was demonstrated that “GH secretory rates and peripheral GH concentrations were maximally correlated with sleep stage, with lags of 4.5 and 16 min, respectively, suggesting that maximal GH release occurs within minutes of the onset of stage 3 or 4 sleep”.
Furthermore “sleep-related augmentation of GH secretion… usually occurs around midnight and the GH levels at that time are, as a rule, at their highest during the 24-hour period. Partially, this phenomenon is time-entrained and partially related to sleep itself. It is associated with a slow wave sleep, and the maximal GH levels occur within minutes of the onset of slow wave sleep” - Holl RW, Hartman ML, Veldhuis JD, et al. Thirty-second sampling of plasma growth hormone in man: correlation with sleep stages. J Clin Endocrinol Metab 1991;72:854–61.
The origin of nocturnal GH release in humans is still unknown. Most likely hypothalamic GHRH release is a major contributing component, but an additional role of another factor, presumably augmenting GHRH responsiveness of the somatotrophs, is likely. However the precise explanatory mechanisms are still not fully identified.
It is worth reiterating though that nocturnal release of GH makes up only a fraction of the total daily GH release in women, but the bulk of GH output in men.
Delta Sleep-inducing Peptide
DSIP (Delta Sleep-inducing Peptide) is used for sleep, it also increases some Growth Hormone output and it's also being used and looked at to prevent opioid addiction.
Gaba & its derivatives
Gamma-amino butyric acid (GABA) is an amino acid and the chief inhibitory neurotransmitter in the central nervous system and the retinas of humans, which regulates muscle tone and other functions. It is also chiefly an excitatory neurotransmitter in most species.
GABA taken orally has been purported to increase the amount of the Human Growth Hormone. One such study demonstrated that gram amounts “induced clear-cut GH increments in plasma with a peak after 60–90 minutes”. The results of those studies have been seldom replicated, and have recently been in question since it is unknown whether GABA can pass the blood-brain barrier. However GABA when taken in injected form has been shown to both pass the blood-brain barrier and have a pronounced GH secretory effect.
At various times & in various studies GABA has been found to both inhibit GH release and exacerbate it. This contradiction is explained as… “inhibition of the GH release stimulated by GABA and its ability to raise baseline GH share the same basic mechanism, i.e., an action through dopaminergic (DA) neurons. Continuous stimulation of central nervous system (CNS-DA) receptors by GABA mimetics through DA release would ultimately lead to a state of partial refractoriness to DA-mediated events…”
The key then appears to be the avoidance of continuous stimulation of the central nervous system.
Two derivatives of GABA, gamma-amino-beta-hydroxy butyric acid (GABOB) and gamma- hydroxy butyric acid (GHB) have been shown to increase GH secretion as well.
PGH (aka Tyric-6)
PGH (Tyric-6) is a gaba-derivative injectable growth hormone stimulant that simply contains:
GABA - a neurotransmitter with a stabilizing role in the brain that has a calming effect. GABA also under certain circumstances increases levels of human growth hormone; and
GABOB - This has been used in the treatment of epilepsy, but it is a legal molecule that is very similar to GHB (a now illegal molecule) without GHBs negative effects; and
Magnesium amino-bromohydrate - An essential mineral which enhances the effect of GABA & GABOB.
The key to understanding why pGH (Tyric-6) is an effective GH releaser is recognizing that GHB (and presumably GABOB as well as GABA in certain circumstances) enhances both slow wave sleep and GH release. - Van Cauter E, Plat L, Scharf MB, et al. Simultaneous stimulation of slow-wave sleep and growth hormone secretion by gamma-hydroxybutyrate in normal young men. J Clin Invest 1997;100:745–53.
While GHB is illegal and not a component of PGH, its similarly structured brother GABOB has been shown to enhance slow wave sleep as well as increase GH. “Significant increases of prolactin and GH plasma levels were observed after injection with 100 mg GABOB”. - Gamma-amino-beta-hydroxy butyric acid stimulates prolactin and growth hormone release in normal women, P Fioretti,… Journal of Clinical Endocrinology & Metabolism, Vol 47, 1336-1340
In another study an intrathecal injection of 300 mg of L-GABOB to cerebrovascular patients caused significant increases in plasma GH, prolactin and cortisol levels at 60 min after injection. These results indicate that GABOB may elicit the secretion of GH, prolactin and ACTH via the central nervous system. - Stimulatory Effects of Gamma-Aminohydroxybutyric Acid (GABOB) on Growth Hormone, Prolactin and Cortisol Release in Man, J. Takahara…
Although the data is sparse and GABA studies as they relate to GH release no longer occur it appears that injectable forms of GABA & its derivatives enhance SWS (slow wave sleep) which is the time period GH is secreted. Since the majority of GH release is secreted in the first early round of SWS it seems that administration of PGH (GABA & GABOB) by injection just prior to sleep will enhance restful sleep and increase the GH secretory pulse.
Over the last few years anectdotal feedback from users including myself report both of these occurances. A protocol involving taking periodic breaks from use appears to be sufficient to promote continued effectiveness.
PGH is a simple, safe and effective compound whose ingredients may be purchased easily over the counter. GABA is sold in most supplement stores, while GABOB is harder to source but is available with international sourcing likely being less expensive.
Of course pGH is available from a research chemical facility and if purchased already assembled need only be reconstituted in Bacteriastatic Water prior to use.
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