Hygetropin Blacktop

[RonnyT Senior Member Find all posts View Profile Close RonnyT]

People can buy dfferent brandnames from different sources. Naked HGH - Strongtropin - Nipertropin - Genetropin - Kigtropin - Riptropin - Nordictropin - Jintropin - Hygetropin. The name and appearance is no longer a warranty for quality, everyone can counterfeit it and often they start with good quality and later turn bad.

In September 2012 I got from a source the than new Hygetropin Blacktop. I asked one of my friends to do an SDS-Page analyses on the than new Blacktops. Now (December 2015) I did ask him again, since as we all know quality differs from batch to batch. I also think that we can learn from comparing analytical results.

First 2012 results

I am using Invitrogen products for running buffer (Novex Tris Glycine), loading buffer, sample reducing agent, electrophoresis unit and precast 4-20% tris-glycine gel.

reconstituted each sample with 1mL of ultra pure water. (theoretically 10iu/vial or 10iu/mL)

sample prep; 2uL of sample + 18 uL of sample buffer which was 4:1 mix of loading buffer/reducing buffer

incubated the samples at 95 degrees Celsius for 5 minutes.

loaded 20uL into each well.

using BioRad Dual Protein marker as reference marker ladder.

160 volt for 1 hour.

washed with DI water twice, and stained for 5 min using Safestain simplyblue from invitrogen. I followed their instructions on washing/staining.
rinsed in DI water for 1 hour.

Image taken by Fujifilm LAS4000
****************************
We knew from analyses in an analytical lab that the browncap hygetropin used as crs (reference) was 13-14 iu
notice a darker impurity band (which is less than 5%) on eli than hygetropin.

the vials were reconstituted using ultra pure water, 1ml per vial, 10iu/vial concentration theoretically.

Hygetropin 0.1176/0.1176 * 100 = 100.0 % = 10iu/vial
elitropin 0.0891 / 0.1176 * 100 = 75.8 % = 7.6iu /vial


Hygetropin 0.1176/0.1176 * 100 = 100.0 % = 14 iu/vial
elitropin 0.0891 / 0.1176 * 100 = 75.8 % = 10.6iu /vial

Hygetropin 14 iu times 0,758 = 10.6 iu

[RonnyT Senior Member Find all posts View Profile Close RonnyT]

results from december 2015

Quality was just a slight bit less. But we are checking rhGH from china a lot and I have seen much worse
These Blacktops now have a website listed on the box and users can check the anti-counterfeit code now. Guess this novelty seems necessary nowadays..
The analyst:

Hi Ronny,

so the first run I overloaded the sample (A) as you can see its just super saturated and of course this also amplified visual of degraded bands and impurities (there are some always)

2nd run (B) , i diluted 20 fold ( I think that was too much lol)
and I ran hygetropin positive control side by side
also did 5 % of the samples

this way, if there are impurity bands, and they are darker than 5% lane, we can say the purity is less than 95%. If the impurity bands are less dark than 5% we can call it purity is greater than 95%

as you can see, even the hygetropin sample shows degradation and there are at least one or two bands extra

your sample is less dark than hygetropin meaning less protein present. However we talked already that old hyge was 13 iu or so anyways,

You can see slight degradation . lets say 90% purity?

I say go for it, looks good to me!!

ps. ive always seen two extra bands of degraded materials from elitropin, good kigtropin, and sometimes kefei in the past. Some other GH also (pharma stuff) showed degradation bands. They are always at the same position as the pics shown. so I am not worried at all. Real impurity bands are way higher on top of the molecular weight from what I have seen in the past. Smaller bands in size than 20-22kDa are just degraded GH frags.

[Jas79 Junior Member Find all posts View Profile Close Jas79]

Quality was just a slight bit less. But we are checking rhGH from china a lot and I have seen much worse
These Blacktops now have a website listed on the box and users can check the anti-counterfeit code now. Guess this novelty seems necessary nowadays..
The analyst:

Hi Ronny,

so the first run I overloaded the sample (A) as you can see its just super saturated and of course this also amplified visual of degraded bands and impurities (there are some always)

2nd run (B) , i diluted 20 fold ( I think that was too much lol)
and I ran hygetropin positive control side by side
also did 5 % of the samples

this way, if there are impurity bands, and they are darker than 5% lane, we can say the purity is less than 95%. If the impurity bands are less dark than 5% we can call it purity is greater than 95%

as you can see, even the hygetropin sample shows degradation and there are at least one or two bands extra

your sample is less dark than hygetropin meaning less protein present. However we talked already that old hyge was 13 iu or so anyways,

You can see slight degradation . lets say 90% purity?

I say go for it, looks good to me!!

ps. ive always seen two extra bands of degraded materials from elitropin, good kigtropin, and sometimes kefei in the past. Some other GH also (pharma stuff) showed degradation bands. They are always at the same position as the pics shown. so I am not worried at all. Real impurity bands are way higher on top of the molecular weight from what I have seen in the past. Smaller bands in size than 20-22kDa are just degraded GH frags.

So how good are they, from 2012 tested to 2015 one tested.

Sorry to add here, if its right place or not as i've been looking at growth hormone and reading a lot of your blog posts, and the growth hormone 4 posts are incredible good piece of work, so thank you! A follow up i had to article 4 when you got the 4 types tested, its written the blue tops had 7.8% related content protein, and the Hygetropin.com.cn had the lowest content of impurities 0.9%. Is this a typo error in the article, should they both be content of impurities and not protein if the hygetropin.com.cn are the cleanest out of the 4 tested? As i am assuming the blue tops are nowhere near as good, and could be dangerous with high impurity content. This isn't a nit-pick but its for my understanding for clarity purpose.

Also, what could this high amount of impurity mean specifically, are we talking something dangerous to human, in the testing that was done. If you could expand if its dangerous, please share, thanks

[RonnyT Senior Member Find all posts View Profile Close RonnyT]

Bro,

Nope, no type error. At that time we tested a counterfeited box, many Hygetropins are just plain counterfeits. Thats why I add pictures and info on appearance.

Sometimes the impurities are no actual impurities, but by example Dimers (2 molecules merged together) weighing double the amount of the single molecule. or trimers etc. Mostly the real impurity is degradation, breakdown of the GH molecules, oc lower in weight than GH. It's the amount of purity that counts. Of course everybody claims his GH to be 99.7% pure, thats why we analyse, to let you know. In the last analyses you know the compound was about 90%. You also can read the comment of the analyst, its not perfect but g2g.

[myosaurus Member Find all posts View Profile Close myosaurus]

as always, we the BB community really appreciate your work done here RonnyT!

[RonnyT Senior Member Find all posts View Profile Close RonnyT]

counterfeits

Thank you for your kind words, bro. Nice to know ones work is appreciated.

We did an very thorough analysis on Chinese GH, but the conclusion was that the browntops produced and distributed by PE where excellent and overdosed. Soon after the news spread through the BB community, a counterfeit was brought to the market, very very cheap, but that was because it contained NO active ingredient what so ever.
I will post some comparison pictures. The same fu** also brought Hygetropin.asia on the market with the same oversized box and “authenticity” seal.

Here a link to the UK that also analysed these products:

I used JINTROPIN of 10IU the Green top which I bought it from Hong Kong pharmacy myself (~265 US$) and Hygetropin 10IU brown top identical with the one in the picture posted above. The Hygetropin 10IU brown top I bought from the same dealer that sells fake Omnitrope and Norditropin cartridges.

Hey Bros, as a heads-up info, the hygetropin with brown tops and hygetropin.asia link to scratch off were tested and have 0 IU HGH inside. They mostly come from former Soviet Union state of Latvia
http://www.uk-muscle.co.uk/muscle-re...-inside-2.html

[RonnyT Senior Member Find all posts View Profile Close RonnyT]

again

Another comparisonpicture that clearly shows the two boxes from the same counterfeiters have the same seals compared to the real one. And also the same reddish brown caps that you can look through compared to the really brown looking real caps. Hope this enlightens it a bit..

[Mr.Wong Member Find all posts View Profile Close Mr.Wong]

Thank you for your kind words, bro. Nice to know ones work is appreciated.

We did an very thorough analysis on Chinese GH, but the conclusion was that the browntops produced and distributed by PE where excellent and overdosed. Soon after the news spread through the BB community, a counterfeit was brought to the market, very very cheap, but that was because it contained NO active ingredient what so ever.
I will post some comparison pictures. The same fu** also brought Hygetropin.asia on the market with the same oversized box and “authenticity” seal.

Here a link to the UK that also analysed these products:

I used JINTROPIN of 10IU the Green top which I bought it from Hong Kong pharmacy myself (~265 US$) and Hygetropin 10IU brown top identical with the one in the picture posted above. The Hygetropin 10IU brown top I bought from the same dealer that sells fake Omnitrope and Norditropin cartridges.

Hey Bros, as a heads-up info, the hygetropin with brown tops and hygetropin.asia link to scratch off were tested and have 0 IU HGH inside. They mostly come from former Soviet Union state of Latvia
http://www.uk-muscle.co.uk/muscle-re...-inside-2.html

RonnyT great job but I've seen you've taken SDS done by other guys and placed the juicedmuscle watermark. Its all cool but I guess in a corner you should mention also the source as that guy might have spend money on this.

[ODB Senior Member Find all posts View Profile Close ODB]

Thank you RonnyT, valuable Info.

[MrBig Senior Member Find all posts View Profile Close MrBig]

Yup, thanks Ronny!

[RonnyT Senior Member Find all posts View Profile Close RonnyT]

RonnyT great job but I've seen you've taken SDS done by other guys and placed the juicedmuscle watermark. Its all cool but I guess in a corner you should mention also the source as that guy might have spend money on this.

I did mention the source (uk-muscle) all others were performed on my request.

[muscleroid Junior Member Find all posts View Profile Close muscleroid]

Good to know this, thank you Ronny