Our American friend Ed is perfecting his analytical skills. He says on these samples and SDS-PAGE:
I made 10 fold dilution of all samples because the bands looked overloaded and too thick previously (7.5mcg etc)
and also included old hygetropin reference sample and 5% of that sample to call out purity based upon intensity but it’s way too faint! hahha
diluted further to make the bands thinner and not too fat and over loaded
im not going to repeat the assay,
but next time i will do 5fold dilution not 10 fold. I think I diluted too much
looks like canada and New Hyge look very similar; size/intensity
Angtropin and Hyge (old reference) look similar
Kefei and blue top look similar; smaller in size and intensity than canada, ang, and hyge however not by much.
very clean single band on sample lanes are shown.
I made 10 fold dilution of all samples because the bands looked overloaded and too thick previously (7.5mcg etc)
and also included old hygetropin reference sample and 5% of that sample to call out purity based upon intensity but it’s way too faint! hahha
diluted further to make the bands thinner and not too fat and over loaded
im not going to repeat the assay,
but next time i will do 5fold dilution not 10 fold. I think I diluted too much
looks like canada and New Hyge look very similar; size/intensity
Angtropin and Hyge (old reference) look similar
Kefei and blue top look similar; smaller in size and intensity than canada, ang, and hyge however not by much.
very clean single band on sample lanes are shown.
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