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interesting info about adipotide (studies from 2011+2012)
http://stm.sciencemag.org/search?journal_search_keyword_go.y=0&submit=yes&fu lltext=adipotide&andorexacttitleabs=and&andorexact fulltext=and&where=fulltext&journal_search_keyword _go.x=0&hopnum=1
A little breakdown from a Dude with a background in biomedical research.
Read the paper by Barnhardt et al on adipotide and can reply to the last few posts. This is a breakthrough for weight loss as it uses an entirely new mechanism of action (killing the blood vessels that support the fat tissue) and does not target the nervous system. It is spectacular science and I suggest you read the paper to see for yourself.
You can get a copy of the paper at ScienceTranslationalMedicine.org for $15. (link above)
First, it does not cause kidney failure as some claim. Here is what the paper says on page 8:
"The primary side effect is relatively
mild, predictable, and reversible renal injury and altered tubular
function, demonstrating that the efficacy of adipotide in highly relevant
primate models of human obesity suggests that it is a promising
candidate drug for translation into clinical applications."
In figure 7 they show images of kidney tubules before, during and after treatment and as you can see the kidneys are not damaged very much by the treatment and they recover completely.
Secondly, it will be 5-10 years before the clinical trials are performed and the drug has FDA approval and gets on the market. They have started human trials in Houston at the Anderson Cancer Center on patients with prostate cancer that might benefit from lower fat levels because it is easier to get approval for trials on cancer patients. This proves that the authorities are convinced by the animal studies that toxicity is not an issue.
On page 5 of the Science paper, they say the following concerning toxicity:
"Although detailed
single- and multiple-dose toxicology studies will be published elsewhere,
here, we report that dose-dependent toxicity in monkeys receiving
single doses of adipotide up to 100 mg/kg (~133-fold of the corresponding
therapeutic dose) did not result in lethality."
In other words, if they give monkeys 133 times the dose needed to lose weight (10-15% of body mass is lost in 60 days after 30 days of treatment) it does not kill them. It sounds quite safe to me.
Concerning availability before FDA approval, that is a grey area and it depends where you are. The University of Texas owns the patent rights which have been licensed to a company in Pasadena, California called Ablaris. It has been patented in the US, the EU and will soon be patented in Canada so if you are not in these countries you can probably make, sell and buy the peptide without breaking the law. There are hundreds of peptide synthesis companies around the world, and the peptide is only 25 amino acids in length and quite easy to synthesize. The cost would be less than $10,000 dollars for 1 gram, enough for a 30 day treatment for one person weighing 100 kg. This is the cost for a custom synthesis of a 25 amino acid peptide purified to 98% and delivered as the acetate salt. A bulk synthesis of 1 kg of the peptide will cause the cost per treatment to drop by at least 20 fold. Once the drug companies start making it, the cost per dose will drop to a few dollars. Its all a matter of scale, and what can be purchased from a peptide lab today is a small scale custom synthesis, so of course the price is very high. Peptide labs make and sell peptides of any sequence all the time and leave it up to the buyer to determine if the sequence is patented, and they keep the orders confidential. Peptides are inherently safe and have not been abused by recreational drug users , so the authorities do not regulate their production.
OK, so if you really wanted to try this new weight loss drug then you can do it. Order the peptide yourself, preferably from a lab that is not located in a country where the patents have been issued, such as Israel, Japan, or India. There are plenty of good peptide labs in these countries. Maybe Mexico if you can find a good lab. Then self administer the peptide to yourself (280 micrograms per kilo per day, subcutaneously in saline solution) in a country where the patents are not issued.
You should do this under a doctor's supervision. You might have to tell him that you are trying a new weight loss treatment, without going into the details, because most doctors will not participate in a treatment that it not approved by the authorities. Have a serum and urine tests done before, during, and after treatment and check for the same parameters that they look at in the monkeys in the science paper. This will cost extra, but is worth it to make sure nothing is going wrong.
If the monkey studies (done on obese rhesus monkeys) is any indication, then you should lose 10-15% of body mass and 40% of body fat in 60 days without any change in lifestyle. The fat, lazy monkeys (all female by the way) continued to be lazy, they just did not eat as much of the high calorie foods. here is what the paper says on page 3:
" Monkeys receiving adipotide remained bright and alert throughout
both experiments. They maintained a consistent level of activity,
interacted with their caretakers appropriately, and presented no overt
clinical signs of nausea, vomiting, or food aversion. Monkeys were fed
a specified amount of commercially produced primate biscuits and
enrichment supplements, and the amount of each food type consumed
was documented daily. All treated and control monkeys consumed
nearly all of their enrichment supplements throughout the
treatment interval; in contrast, the number of monkey chow biscuits
consumed by treated monkeys decreased in an inversely proportional
manner with the dose of adipotide administered and/or the amount of
weight lost over time (Fig. 5C and fig. S3)."
FDA is very cautious (overly cautious in my opinion) when approving new weight loss drugs and it will be many years before it becomes available.
Whatever you do, do not buy "adipotide" online. It will not be the real thing. If the seller insists it is, ask to see the HPLC results and the mass spectra, which all legitimate peptide synthesis labs provide with their products. The mass should be about 2,557 Daltons.
The sequence is: CKGGRAKDC-GG-D(KLAKLAK)2 - (but this is not enough. The peptide must be cyclized using the 2 cysteines (the Cs in the sequence) at positions 1 and 9 and this requires more work. Without the cyclization the peptide will rapidly be degraded by proteases in the blood and will be useless. You will need hundreds of times more peptide if it is not cyclized and this is a no-starter. GWP claims that they provide mass spectrometry results on all of their peptides which can be used to determine if the correct cyclization has been done. The cyclization will reduce the mass of the peptide by exactly 2 Daltons because 2 hydrogen atoms are released when the sulfurs in the cysteines bond.
The monoisotopic mass of the cyclized peptide is 2553.514 Daltons and can be verified by any lab that has a mass spectrometer. So if GWP starts selling adipotide, you can be sure that I will buy some and check the mass myself and post it here. Even though GWP sells their peptides for research purposes only, this community cannot tolerate anyone selling a peptide as "adipotide" unless it has the same chemistry as the adipotide peptide decribed in the literature, which as we all know showed spectacular weight loss in Rhesus monkeys.).
more;
The estimated dose for humans is 280 ug per kg per day. If you weigh 100 kg then you will need 28 mg per day. You will probably need to take the treatment at least 30 days, so this works out to 840 mg for the treatment (28 x 30). If you order 840 mg and want to inject 1 ml of solution per day (1 ml is a convenient amount to inject subcutaneously) then you should dissolve the 840 mg in 30 ml of saline and inject 1 ml per day.
The FDA approved trials in prostate cancer patients that are planned to start in January, 2012 in Houston, Tx will give the patients only 30 micrograms per kg per day. This is because they don't know for sure that 280 ug/kg/day is a safe dose in humans so they are starting the first human trials with much less (almost 10 times less). They will be looking for side effects, including kidney damage. Once they determine that 30 ug/kg/day is safe then they will increase the dose. The whole process will take years and this is why Adipotide will not be FDA approved for many years (7 to 10 in my estimate)."GYM + JUICE" -
Yup and I'm sure we haven't seen the last of it, its extremely expensive and in Germany and Austria people are trying "in vivo" studies that are very promising.
Thanks for the info, bro..Comment
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