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The calcium flux cAMP assay for GPCR signaling is a critical technique in studying G protein-coupled receptor (GPCR) activity. By measuring changes in calcium and cyclic AMP levels, researchers can investigate GPCR-mediated signaling pathways with high precision. This method is widely used in drug discovery to screen compounds targeting GPCRs, which are involved in numerous physiological processes. The calcium flux cAMP assay for GPCR signaling provides valuable insights into receptor function and aids in the development of novel therapeutics.