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I would like to be able to analyse inside China and we are using such facilities right now. One is busy buying all needed equipment, the other one is allready operational.
They are trying to find a quick and cheap way to analyse several batches for strength and purity. SDS-PAGE is such method. For smaller peptides such as IGF-1 LR3, SDS-PAGE is not suitable, since its molecular weight is so small, only 83 amino acids. exerpt:
For those interested in the MOA:
RP-HPLC was performed on a Kromasil C4 column with 250 mm length and 4.6 mm internal diameter, a particle size of 5 μm and a pore size of 300 Angstrom. As mobile phase a mixture of 29 volumes of propanol and 71 volumes of 50 mM tris-hydrochloride buffer solution pH 7.5 was used in isocratic mode at a flow rate of 0.5 ml/min. The column temperature was maintained at 45 °C. UV detection w as at the wavelength of 220 nm. 20 μl (40 μg) of the sample standard were injected onto the column and the area of the single peak eluting at a retention time of around 25 min served as reference for the content of
the other samples.
They are trying to find a quick and cheap way to analyse several batches for strength and purity. SDS-PAGE is such method. For smaller peptides such as IGF-1 LR3, SDS-PAGE is not suitable, since its molecular weight is so small, only 83 amino acids. exerpt:
For those interested in the MOA:
RP-HPLC was performed on a Kromasil C4 column with 250 mm length and 4.6 mm internal diameter, a particle size of 5 μm and a pore size of 300 Angstrom. As mobile phase a mixture of 29 volumes of propanol and 71 volumes of 50 mM tris-hydrochloride buffer solution pH 7.5 was used in isocratic mode at a flow rate of 0.5 ml/min. The column temperature was maintained at 45 °C. UV detection w as at the wavelength of 220 nm. 20 μl (40 μg) of the sample standard were injected onto the column and the area of the single peak eluting at a retention time of around 25 min served as reference for the content of
the other samples.