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Nowadays, it is widely accepted that HS is an information-rich polymer and alteration in HS expression has been associated with the disease. However, the structure-function relationships of HS and related proteins are very poorly understood due to the chemical complexity and heterogeneity of HS. The development of HS microarray can substantially improve the understanding of HS-protein interactions, opening an opportunity for the discovery of novel therapeutic interventions for a variety of disease states.